Test No. 321: Hyallela Azteca Bioconcentration Test (HYBIT)
The Hyalella azteca Bioconcentration Test (HYBIT) provides a non-vertebrate test for
bioconcentration in aquatic environments. The test consists of two phases: the exposure
(uptake) and post-exposure (depuration) phases. During the uptake phase, a group of
H. azteca is exposed to the test chemical at one or more chosen concentrations. They
are then transferred to a medium free of the test chemical for the depuration phase.
The concentration of the test chemical in the analysed H. azteca is followed through
both phases of the test. Parameters which characterise the bioaccumulation potential
include the uptake rate constant (k1), the depuration rate constant (k2), the steady-state
bioconcentration factor (BCFSS) and the kinetic bioconcentration factor (BCFK).
Published on June 25, 2024Also available in: French
In series:OECD Guidelines for the Testing of Chemicals, Section 3: Environmental fate and behaviourview more titles