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The present Key Event based Test Guideline addresses the human health hazard endpoint skin sensitisation, following exposure to a test chemical. Skin sensitisation refers to an allergic response following skin contact with a tested chemical, as defined by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). This Test Guideline is proposed to address a Key Event leading to skin sensitisation, namely keratinocyte activation. This Key Event on the Adverse Outcome Pathway (AOP) leading to skin sensitisation takes place in the keratinocytes and includes inflammatory responses as well as gene expression associated with specific cell signalling pathways such as the antioxidant/electrophile response element (ARE)-dependent pathways. This Test Guideline provides three in vitro test methods addressing the same Key Event on the AOP for skin sensitisation: (i) the ARE-Nrf2 luciferase KeratinoSens™ test method, (ii) the ARE-Nrf2 luciferase LuSens test method and (iii) the Epidermal Sensitisation Assay – EpiSensA. The KeratinoSens and the Lusens are in vitro ARE-Nrf2 luciferase-based test methods and the EpiSensA is based on gene expression quantification using Reverse Transcription- quantitative PCR in reconstructed human epidermis models. The proposed test methods are used for supporting the discrimination between skin sensitisers and non-sensitisers in accordance with the UN GHS. Performance standards have been developed to enable the validation of similar test methods.

The in vitro macromolecular test method is a biochemical in vitro test method that can be used to identify chemicals (substances and mixtures) that have the potential to induce serious eye damage as well as chemicals not requiring classification for eye irritation or serious eye damage. The in vitro macromolecular test method contains a macromolecular reagent composed of a mixture of proteins, glycoproteins, carbohydrates, lipids and low molecular weight components, that when rehydrated forms a complex macromolecular matrix which mimics the highly ordered structure of the transparent cornea. Corneal opacity is described as the most important driver for classification of eye hazard. Test chemicals producing protein denaturation, unfolding and changes in conformation will lead to the disruption and disaggregation of the highly organised macromolecular reagent matrix, and produce turbidity of the macromolecular reagent. Such phenomena is quantified, by measuring the changes in light scattering (at a wavelength of 405 nm using a spectrometer), which is compared to the standard curve established in parallel by measuring the increase in OD produced by a set of calibration substances.

This method provides information on health hazard likely to arise from short-term exposure to a test article (gas, vapour or aerosol/particulate test article) by inhalation. The revised Test Guideline describes two studies: a traditional LC50 protocol and a Concentration x Time (C x t) protocol. It can be used to estimate a median lethal concentration (LC50), non-lethal threshold concentration (LC01) and slope, and to identify possible sex susceptibility. This Test Guideline enables a test article quantitative risk assessment and classification according to the Globally Harmonized System for the Classification and Labelling of Chemicals. In the traditional LC50 protocol, animals are exposed to one limit concentration or to three concentrations, at least, for a predetermined duration, generally of 4 hours. Usually 10 animals should be used for each concentration. In the C x T protocol, animals are exposed to one limit concentration or a series of concentrations over multiple time durations. Usually 2 animals per C x t interval are used. Animals (the preferred species is the rat) should be observed for at least 14 days. The study includes measurements (including weighing), daily and detailed observations, as well as gross necropsy.

This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology for human recombinant in vitro assays to detect substances with estrogen receptor binding affinity (hrER binding assays). It comprises two mechanistically and functionally similar test methods for the identification of estrogen receptor (i.e. ERα) binders and should facilitate the development of new similar or modified test methods. The two reference test methods that provide the basis for this PBTG are: the Freyberger-Wilson (FW) In Vitro Estrogen Receptor (ER) Binding Assay Using a Full Length Human Recombinant ERα, and the Chemical Evaluation and Research Institute (CERI) In Vitro Estrogen Receptor Binding Assay Using a Human Recombinant Ligand Binding Domain Protein. This assay measures the ability of a radiolabeled ligand ([3H]17β-estradiol) to bind with the ER in the presence of increasing concentrations of a test chemical (i.e. competitor).  Test chemicals that possess a high affinity for the ER compete with the radiolabeled ligand at a lower concentration as compared with those chemicals with lower affinity for the receptor. This assay consists of two major components: a saturation binding experiment to characterise receptor-ligand interaction parameters and document ER specificity, followed by a competitive binding experiment that characterises the competition between a test chemical and a radiolabeled ligand for binding to the ER. These test methods are being proposed for screening and prioritisation purposes, but also provide mechanistic information that can be used in a weight of evidence approach.

A circular economy keeps the value of resources in the economy for longer, extends the useful lifespan of products and reduces waste, thereby reducing environmental and climatic pressures and increasing domestic competitiveness. Italy is among the leading European actors in transitioning to a circular economy. Its adoption of the National Strategy for the Circular Economy in 2022 reinforced the country’s ambition to rapidly shift from linear to circular modes of production and consumption. Among the envisioned measures, the national strategy calls for a stronger use of economic instruments to achieve a more coherent and effective policy mix. This report identifies opportunities for the enhanced use of economic instruments to support the circular economy in Italy. Part I of this report takes stock of the Italian policy landscape, compares it to international practices and recommends seven policy reforms for further consideration. Part II contains an in-depth analysis of three policy instruments that could reduce demand for virgin materials and promote a shift to secondary materials. These instruments include a virgin materials tax on construction minerals, a reduced VAT rate for products with recycled content and corporate tax credits to promote the use of secondary materials.

The frequency and severity of extreme wildfires are on the rise in Greece, causing unprecedented disruption and increasingly challenging the country’s capacity to contain losses and damages. These challenges are set to keep growing in the context of climate change, highlighting the need to scale up wildfire prevention and climate change adaptation. This paper provides an overview of Greece's wildfire policies and practices and assesses the extent to which wildfire management in the country is evolving to adapt to growing wildfire risk under climate change.

Greenhouse gas (GHG) emissions data is essential for tracking progress towards the Paris Agreement’s global temperature goals. In addition to the emissions inventories based on the Intergovernmental Panel on Climate Change guidelines, which are used for tracking progress under the framework of the Paris Agreement, there are other GHG emissions datasets that cater to different users and policy objectives. This paper evaluates three OECD datasets on GHG emissions: Air emissions – Greenhouse gas emissions inventories, Agricultural greenhouse gases emissions, and the Air Emissions Accounts (AEAs) based on the System of Environmental-Economic Accounting. It also looks at one dataset from the International Energy Agency: CO2 emissions from fuel combustion. It discusses the inventory and AEA approaches in terms of accounting principles (production- versus demand-based, territory versus residence), as well as classifications and scope of emissions. It looks at the coverage of the GHG emissions datasets and identifies the data gaps. Finally, the paper outlines several steps to enhance data coverage and quality of the datasets.

Thailand has adopted ambitious clean energy targets to meet its long-term climate goals, committing to reach carbon neutrality by 2050 and net zero greenhouse gas (GHG) emissions by 2065. Transforming Thailand’s energy system, alongside broader development objectives, is critical to meeting these goals as the energy sector accounts for 69% of Thailand’s total GHG emissions. The Clean Energy Finance and Investment Roadmap of Thailand ('the Roadmap') outlines key actions to unlock finance and investment in two clean energy sectors: (i) renewable power, with special attention to small-scale renewable power systems; and (ii) energy efficiency in buildings, with a focus on cooling applications. The two sectors were selected in close consultation with the Department of Alternative Energy Development and Efficiency (DEDE) of the Ministry of Energy of Thailand. The Roadmap provides a comprehensive overview of the progress to date, policy context and challenges to mobilise near-term finance in those sectors, as well as estimates of the finance needs to reach Thailand’s clean energy plans. The report also includes a roadmap action plan, suggesting non-prescriptive recommendations and actions that the Government of Thailand, financial institutions, energy service companies, academia and the international development community active in the country could undertake to foster clean energy investments in Thailand.

The report outlines the aggregate trends in annual climate finance provided and mobilised by developed countries for climate action in developing countries over the period 2013-2022. It presents these trends by source of finance, climate theme, sector, income group and type of financial instrument. Additionally, the report explores adaptation finance trends in relation to the Glasgow Pact's call for developed countries to double adaptation finance by 2025 and offers insights into the growing significance of multilateral providers in the climate finance landscape.