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  • 8-September-2009

    English

    Test No. 232: Collembolan Reproduction Test in Soil

    This Test Guideline is designed for assessing the effects of chemicals on the reproduction of collembolans in soil. The parthenogenetic Folsomia candida is the recommended species for use, but an alternative species such as sexually reproducing Folsomia fimetaria could also be used if they meet the validity criteria. This Guideline can be used for testing both water soluble and insoluble substances but it is not applicable to volatile ones. The Guideline aims to determine toxic effects of the test substance on adult mortality and reproductive output expressed as LCx and ECx respectively, or NOEC/LOEC value. The number of treatment concentrations varies depending on endpoints to be determined. For a combined approach to examine both the NOEC/LOEC and ECx, eight concentrations in a geometric series with four replicates for each concentration as well as eight control replicates should be used. In each test vessel, 10 juveniles F. candida (or 10 males and 10 females adults F. fimetaria) should be placed on 30 g of modified OECD artificial soil using a 5 % organic matter content. The duration of a definitive reproduction test is 4 weeks for F. candida or 3 weeks for F. fimetaria.

  • 16-October-2008

    English

    Test No. 226: Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil

    This Test Guideline describes a method to assess the effects of chemical substances in soil on the reproductive output of the soil mite species Hypoaspis (Geolaelaps) aculeifer Canestrini (Acari: Laelapidae). It can be used for water soluble or insoluble substances, but not with volatile substances. Adult females of similar age are exposed to a range of concentrations of the test substance mixed into 20 g dry mass of artificial soil 28-35 days after the start of the egg laying period. Depending on the endpoint (ECx, NOEC or both), five to twelve concentrations should be tested. At least two to four replicates for each test concentrations and six to eight control replicates, of 10 animals each, are recommended. At 20¡ãC, the test lasts 14 days after introducing the females, which usually allows the control offspring to reach the deutonymph stage. The number of surviving females (mortality ¡Ü 20% for a valid test) and the number of juveniles per test vessel (at least 50 for a valid test) are determined. The fecundity of the mites exposed to the test substance is compared to that of controls in order to determine the ECx (e.g. EC10, EC50) or the No Observed Effect Concentration (NOEC). Any observed differences between the behaviour and the morphology of the mites in the control and the treated vessels should be recorded.

  • 16-October-2008

    English

    Test No. 228: Determination of Developmental Toxicity of a Test Chemical to Dipteran Dung Flies(Scathophaga stercoraria L. (Scathophagidae), Musca autumnalis De Geer (Muscidae))

    This Test Guideline describes a method to estimate the developmental toxicity of a test chemical to the dung dwelling life stages of dung-dependent dipteran species. Two test species can be used. The test chemical is mixed with bovine faeces, to which either 10 eggs of Scathophaga stercoraria or 10 larvae of Musca autumnalis are added. The test will be terminated 5 days after emergence of the last adult in the control (> 18 days for S. stercoraria, >13 days for M. autumnalis). Then the possible impacts of the test chemical on the following measurement endpoints are assessed under controlled conditions: sex and total number of emerged adult flies, retardation of emergence indicated by the developmental rate and morphological change. Depending on the experimental design, the No Observed Effect Concentration (NOEC) or the Effect concentration for x percent effect (ECx) can be determined. This Guideline can be used for water soluble or insoluble substances, but is not applicable to volatile substances. If the toxicity of the chemical is unknown, five nominal test concentrations should be conducted. A positive control should be tested periodically. The test is considered valid if in the controls hatching of larvae is superior or equal to 70% of the number of introduced eggs, emergence of adults is superior or equal to 70% and superior or equal to 50% of the respectively hatched and introduced larvae and if the emergence of adult flies starts after 18 +- 2 days (S. stercoraria) or after 13 +- 2 days(M. autumnalis).

  • 14-August-2007

    English

    Guidance Document on Pesticide Residue Analytical Methods

    This document provides guidance on the residue analytical methods. Analytical methods are used to generate the data for estimating dietary exposure assessments, to establish Maximum Residue Levels (MRLs), and to determine processing factors.

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  • 23-December-2005

    English

    Good Laboratory Practice: OECD Principles and Guidance for Compliance Monitoring

    This for-sale publication unites all 14 of the OECD documents related to Good Laboratory Practice and compliance monitoring, and, in the Annex, reproduces the three OECD Council Decisions related to the Mutual Acceptance of Data in the Assessment of Chemicals.

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  • 24-July-2002

    English

    Guidance Notes for Analysis and Evaluation of Chronic Toxicity and Carcinogenicity Studies

    This document is intended as a general guide to the analysis and evaluation of data from chronic exposures of toxicity test species to pesticides and other chemicals. It also outlines the kind of information an independent assessment of toxicity studies should encompass.

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  • 23-January-1998

    English

    Council Decision on the Adherence of Non-Member Countries to the Council Acts Related to the Mutual Acceptance of Data in the Assessment of Chemicals

    Since 1997 a procedure through which non-OECD countries can adhere to the MAD system has been embodied in a Council Decision (Council Decision on the Adherence of Non-Member Countries to the Council Acts Related to the Mutual Acceptance of Data i...

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  • 23-January-1998

    English

    Council Decision n the Adherence of Non-Member Countries to the COuncil Acts Related to the Mutual Accpetance of Data in the Assessment of Chemicals

    Since 1997 a procedure through which non-OECD countries can adhere to the MAD system has been embodied in a Council Decision (Council Decision on the Adherence of Non-Member Countries to the Council Acts Related to the Mutual Acceptance of Data i...

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  • 2-October-1989

    English

    Decision-Recommendation of the Council on Compliance with Principles of Good Laboratory Practice [C(89)87/FINAL]

    Decides that Member countries in which testing of chemicals for purposes of assessment related to the protection of health and the environment is being carried out pursuant to principles of good laboratory practice that are consistent with the OECD Principles of Good Laboratory Practice.

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  • 8-December-1982

    English

    Decision of the Council concerning the Minimum Pre-Marketing Set of Data in the Assessment of Chemicals [C(82)196/FINAL]

    Decides that in Member countries sufficient information on the properties of new chemicals should be available before they are marketed to ensure that a meaningful assessment of hazard to man and the environment can be carried out.

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