Test No. 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
This Test Guideline describes a chronic oral toxicity test on adult worker honey bees
under laboratory conditions over an exposure period of 10 days.
Young bees (max. 2 days old) are exposed to 50 % (w/v) aqueous sucrose solution containing
the test chemical by continuous and ad libitum feeding over a period of 10 days. Mortality
and behavioural abnormalities are observed and recorded daily during the 10 day test
period. The chronic effects of the test chemical are evaluated by comparing the results
of the test chemical treated group to those of the respective control group. The test
is designed for the determination of the following endpoints LC50 (median Lethal
Concentration) and the LDD50 (median Lethal Dietary Dose) values after 10 days of
exposure, and NOEC (No Observed Effect Concentration) and NOEDD (No Observed Effect
Dietary Dose).
Published on October 09, 2017Also available in: French
In series:OECD Guidelines for the Testing of Chemicals, Section 2: Effects on Biotic Systemsview more titles